The preventive and curative effect of Honey on the cellular Blood components in CCl4 induced Toxicity in Male Rabbits.

Abstract

The aim of the present study was to investigate the ability of sider honey to protect blood cells from the toxic effect of ccl₄ in male rabbits. For this goal thirty local breed male rabbits (weighted 1000-1400gr) were used. The animals were randomly divided into five groups: every group contain (6) animals. The first group served as control animals, and they orally received only 5ml of diswater once a day for a period of 30 days. The second group were i.m-injected by (1ml/kg) of CCl4 twice a week in order to induce the toxicity in blood cells then they had not treatment with Seder honey for a period of 30 days. Third group were i.m injected by (1ml/kg) of ccl4 twice a week and then they orally received a (1g/kg) of honey dose daily for a period of 30 days. Fourth group of animals were i.m injected by (1ml/kg) of CCl4 a twice-in week and then orally received (2g/kg) of honey dose daily for a period of 30 days. The five group received (2g/kg) of honey for a period of 15 days. In the day of 16^th they were i.m injected (1m/kg) of ccl4 twice a week in order to induce the toxicity in blood cells, with the continuation of receiving the dose of honey until the day of thirty.

     The values of Red Blood Cells (RBC), white Blood cells (WBC) and Haemoglobin (Hb) in blood were estimated. Result showed a significant (p<0.05) decrease in all studied parameters, our results emphasized the high toxicity of (CCl4). Led the use of honey as curative and protective agent to protect the toxic effect of ccl4, and increase the level of (RBC, WBC, Hb) in blood animals. The best results was recorded in 5^th group animals were received (2g/kg) of honey as protective agent, the values of RBC, WBC, Hb were nearly to normal values in control.